Physiological and Agronomical Responses of Triticale to the Growth Bacteria, Nano Iron Fertilizer and Ordinary Iron Fertilizer Treatment


1 Department of Agriculture, Firoozabad Branch, Islamic Azad University, Firoozabad, Iran.

2 Department of Agriculture, Shiraz Branch, Islamic Azad University, Shiraz, Iran.


BACKGROUND: Bio-fertilizers are best eco-friendly approach for plant and soil environment. Also, micronutrient element such as iron plays a key role in the formation of chlorophyll and photosynthesis.
OBJECTIVES: This study evaluated the effect of growth bacteria and Nano iron fertilizer and ordinary iron fertilizer on some physiological properties of Triticale.
METHODS: The experiment was conducted as 4×6 factorial in RCBD design with three replications at two years (2016-2017). Treatments included: Use of plant growth-promoting bacteria in four levels (Non-inoculation, inoculation with Azotobacter crocococcus, Azospirillium methylpofrome and Pseudomonas putida) and nano iron fertilizer in five levels (0, 0.5%, 1%, 1.5% and 2%) and ordinary iron fertilizer on two levels (2% and 0%). According to results it was founded that application of Azotobacter crocococcus, Pseudomonas putida and Azospirillium methylpofrome led to15, 13 and 17% the increase of seed yield by the compare to control. In relation to Nano-Fe fertilizer, it was observed that 1% Nano-Fe fertilizer showed highest seed yield.
RESULT: The results of means comparisons for bacterial treatment showed that the highest leaf area index was related to Azotobacter treatment and the lowest amount was related to control treatment. Regarding the crop growth rate index, the results of the data analysis indicated that bacterial treatment and treatment of iron were significant at 1% level, but the interaction treatment was not significant.
CONCLUSION: Between three tested bacteria, the Azotobacter highest effects on LAI, CGR and HI, so, we propose application of nano-Fe fertilizer and plant growth-promoting bacteria together for the improving Triticale yield in Iran and similar regions. 


Akram, M. S., M. A. Cheema, M. Waqas, M. Bilal. and M. Saeed. 2020. Role of Bio-Fertilizers in Sustainable Agriculture. Al-Amri, Norah, Huseyin Tombuloglu, Yassine Slimani, Sultan Akhtar, Mohammad Barghouthi, Munirah Almessiere, Thamer Alshammari et al. "Size effect of iron (III) oxide nanomaterials on the growth, and their uptake and translocation in common wheat (Triticum aestivum L.)." Ecotoxicol. Environ. Safety. 194 (2020): 110377.

Boomsma, C. R. and T. J. Vyn. 2008. Maize drought tolerance: Potential improvements through Arbuscular mycorrhizal symbiosis. Field Crops Res. 108: 14-31.

Bumandalai, O. and R. Tserennadmid. 2019. Effect of Chlorella vulgaris as a biofertilizer on germination of tomato and cucumber seeds. Intl. J. Aquatic Biol. 7: 95-99.

Buttery, B. R. 1970. Effect of variation in leaf area index on the growth of maize and soybean. Crop Sci. 10: 9-13.

Burhan, M. G. and S. A. AL-Hassan. 2019. Impact of nano NPK fertilizers to correlation between productivity, quality and flag leaf of bread wheat varieties. Iraqi J. Agri. Sci. 50: 1-7.

Enyi, B. A. C. 1962. Comparative growth rates of upland and swamp rice varieties. Ann. Bot. 26: 467-487.

Esitken, A. 2011. Use of plant growth promoting rhizobacteria in horticultural crops, Bacteria in Agrobiology: Crop Ecosystems. Springer. Berlin. Heidelberg. Germany.

Flores-Núñez, V. M., E. Amora-Lazcano, A. Rodríguez-Dorantes, J. A. Cruz-Maya. and J. Jan-Roblero. 2018. Comparison of plant growth-promoting rhizobacteria in a pine forest soil and an agricultural soil. Soil Res. 56: 346-355.

Hassanein, M., Z. Lari. and N. El-Sheimy. 2018. A new vegetation segmentation approach for cropped fields based on threshold detection from hue histograms. Sensors. 18(4): 1253.

Ji, S. H., J. S. Kim, C.H. Lee, H. S. Seo, S. C. Chun, J. Oh, E. H. Choi. and G. Park. 2019. Enhancement of vitality and activity of a plant growth-promoting bacteria (PGPB) by atmospheric pressure non-thermal plasma. Scientific Reports. 9: 1-16.

Khattree, R. D. N. and Naik. 2018. Applied multivariate statistics with SAS software. SAS Institute Inc.

Khoshgoftarmanesh, A. H., R. Schulin, R. L. Chaney, B. Daneshbakhsh. and M. Afyuni. 2010. Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review. Agron. Sustainable Development. 30: 83-107.

Kilpeläinen, J., A. Barbero-López, B. Adamczyk, P. J. Aphalo. and T. Lehto. 2019. Morphological and ecophysiological root and leaf traits in ectomycorrhizal, arbuscular mycorrhizal and non mycorrhizal Alnus incana seedlings. Plant Soil. 436: 283-297.

Omara, A., F. Hauka, A. Afify, M. N. El-Din. and M. Kassem. 2017. The role of some PGPR strains to biocontrol Rhizoctonia solani in soybean and enhancement the growth dynamics and seed yield. Environ. Biol. Soil Security. 1: 47-59.

Rajput, A., S. S. Rajput. and G. Jha. 2017. Physiological parameters leaf area index, crop growth rate, relative growth rate and net assimilation rate of different varieties of rice grown under different planting geometries and depths in SRI. Int. J. Pure App. Biosci. 5(1): 362-367.

Riaz, U., S. M. Mehdi, S. Iqbal, H. I. Khalid, A. A. Qadir, W. Anum, M. Ahmad. and G. Murtaza. 2020. Bio-fertilizers: Eco-Friendly approach for plant and soil environment. In: Bioremediation and Biotechnology. Springer. Cham. pp. 189-213.

Sharif, M. O. and C. S. Shin. 2020. Effect of fertilization and bacterial inoculation on the growth of alder (Alnus sibirica) in coal mine soil. Asian Res. J. Agri. pp. 39-45.

Singh, S., S. Mahabalram. and D. P. Singh. 2008. Agronomic traits contributing to drought tolerance in husk less barley. Rachis: 5: 12-13.

Vyas, P. and R. Kaur. 2019. Culturable stress-tolerant plant growth-promoting bacterial endophytes associated with Adhatoda vasica. J. Soil Sci. Plant Nutr. 19: 290–298.

Wu, S. C., H. Cao. and K. C. Cheung. 2005. Effect of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma. 125: 155-166.

Yasari, E. and A. M. Patwardhan. 2007. Effects of Azotobacter and Azospirillum inoculation and chemical fertilizers on growth and productivity of canola. Asian J.  Plant Sci. 6: 77-82.

Zaied, K., A. H. Abd-EI-Hady, A. H., Afify. M. A. Nassef. 2003. Yield and nitrogen assimilation of winter wheat inoculated with new recombinant inoculant of rhizobacteria. Pak. J. Biol. Sci. 6: 344-358.