Determination Sodium Hypochlorite (NaClO) Application on Quantitative Parameters of Wheat (Triticum aestivum L.) Seed and Correlation Between Measured traits


Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.


To assessment effect of concentration and times of disinfection wheat seeds, infected by Aspergillus fungus a research was conducted based on Factorial experiment according randomized complete block design with four replications. The factors included four levels of sodium hypochlorite (2, 4, 6, and 8%) and four times of seed disinfection (2, 5, 7, and 10 minutes). Result of analysis of variance indicated interaction effect of treatments on germination percentage (p≤0.05), Radicle length (p≤0.05), plumule lentgh (p≤0.05), germination rate (p≤0.05), seed dry matter (p≤0.01), speed of germination (p≤0.05) and seedling dry matter (p≤0.01) was significant, but on mean of germination time, radicle dry weight, plumule dry weight and abnormal bud was not significant. Germination rate as important trait had significant relation with seedling dry matter (0.992**), radicle length (0.990**), germination percentage (0.983**), plumule length (0.982**), speed of germination (0.981**), seed dry matter (0.924**) and abnormal bud (-0.767*). Treatment of 6% concentrations of sodium hypochlorite along 7 minute had highest amount of Seed dry matter (0.0899 gr), speed of germination (38.991 day), seedling dry matter (0.1899 gr), germination percentage (98.87 %), radicle length (11.40 cm), plumule length (10.91 cm), germination rate (39.98 day), so it possible to advised for improve wheat seed germination parameters.


Ajdukovia, K. T. and D. M. Vasia. 2005. Different sterilization methods for overcoming internal bacterial infection in sunflower seeds. Proc. Nat. Sci. Novi. J. 109: 59-64.

Carisse, O., A. Ouimet. and V. Toussaint. 2000. Evaluation effect of seed treatment, bactericides, and cultivars on bacterial leaf spot of lettuce caused by Xanthomonas camprstris. J. Plant Disease. 84: 295-299.

Ghodrat, V. and M. J. Rousta. 2012. Effect of priming with gibberellins Acid (GA3) on germination and growth of corn under saline conditions. Intl. J. Agri. Crop Sci. 4(13): 882-885.

Hasan, M. M., S. P. Chowdhury, A. Shahidul, B. Hossain. and M. S. Alam. 2013. Antifungal effects of plant extracts on seed-borne fungi of wheat seed regarding seed germination. Pak. J. Biol. Sci. 8: 1284–1289.

Ishfaq, S., S. D. Ahmed, A. H. Shah, R. T. Khan, S. M. Fiaz Bukhari, I. Hameed, H. Mubeen, N. Awan, S. R. Abbas. and Sh. Raza. 2016. In-Vitro optimization protocol of wheat cultivars in newly established lab of plant tissue culture, muzaffarabad. Euro. J. Pharm. Medical Res. 3(3): 477-479.

Mendes De-Jesus, V. A., E. F. Araujo, A. A. Neves, F. L. Santos, L. A. D. Santos Dias, R. F. Da-Silva. 2016. Ratio of seeds and sodium hypochlorite solution on the germination process of papaya seeds. J. Seed Sci. 38(1): 57-61.

Miche, L. and J. Balandreau. 2001. Effects of rice seed surface sterilization with hypochlorite on inoculated seeds. App. Environ. Sci. J. 7: 3046–3052.

Musgrave, M. E. and N. Ding. 1998. Evaluating wheat cultivars to water logging tolerance. Crop Sci. J. 34: 90-97.

Pernezny, K., R. Nagata, R. N. Raid, J. Collins. and A. Carroll. 2002. Investigation of seed treatments of management of bacterial leaf spot of lettuce. J. Plant Disease. 7: 151-155.

Qarineh, M. H. 2003. Eco-physiological water stress and growth stage evaluation on durum and bread wheat seeds quality. PhD. Theses. Tabriz Univ. pp: 223.

Razavi, S. A. 2007. Antagonistic effect of Pseudomonas Fluorescens and Trichoderma on Root and seed of Cotton. J. Sci. Tech. Agric. Nat. Res. 42:11-20.

Soltani, A., E. Zeinali, S. Galeshi .and N. Latifi. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from Caspian Sea coast of Iran. Seed Sci. Tech. J. 29: 653-662.