Evaluation Effectiveness of Chemical and Biological Fertilizers Combination on Corn (Zea mays L.) Yield


Department of Agronomy, Khuzestan Science and Research Branch, Islamic Azad University, Ahvaz, Iran.


In order to investigate the appropriate combination of chemical and bio-fertilizers and their effectiveness on corn seed yield while defoliating the upper leaves of the ear, a randomized complete block design experiment with four replications. Fertilizer treatments were applied at four levels including 100% urea, 75% urea + 100% Nitroxin, 50% urea + 100% Nitroxin, 25% urea + 100% Nitroxin. In harvest stage the traits such as seed yield, biological yield, harvest index, 1000-seed weight, number of seeds per row, number of rows per ear, and number of seeds per ear were calculated. The results showed that the effect of fertilizer combination on studied traits was not significant. Although there was no significant difference between the means of seed yield, number of row per ear, number of seed per row, number of seed per ear, and biological yield, the maximum rate of traits belonged to the treatment with 50% urea + 100% Nitroxin by 11.52 t.ha-1, 14.82, 36.85, 547.59, and 21.30 t.ha-1, respectively. In general results showed that application of bio-fertilizer in corn even when the upper leaves of ear are defoliated along with optimum consumption of chemical fertilizers improve yield and yield components. 


Afkhami-Fathabad, Sh., B. Abbaszadeh. and K. Khavazi. 2014. Investigation the quality and quantity of corn under chemical and bio-fertilizers of sulfur. Intl. J. Bio. Sci. 4(4): 92-96.

Afsharmanesh, G.R. 2007. The effect of planting date on seed yield of corn cultivar in early spring planting in Jiroft. Res. Construct. Agri. Hort. 75: 2-8.

Azimzadeh, S. M. and S. J. Azimzadeh. 2013. Effect of nitroxin bio-fertilizer and nitrogen chemical fertilizer on yield and yield components of rapeseed. Intl. J. Agric. Crop Sci. 6(18): 1284-1291.

Biari, A., A. Gholami. and H. A. Rahmani. 2008. Growth promotion and enhanced nutrient uptake of corn by application of plant growth promoting rhizobacteria in arid region of Iran. J. Biol. Sci. 8: 1015-1020.

Boraste, A. 2009. Bio-fertilizer: a novel tool for agriculture. Intl. J. Microbial. Res. 1(2): 23-31.

Burton, J. W., D. W. Israel, R. F. Wilson. and T. E. Carter. 1995. Effects of defoliation on seed protein concentration in normal and high protein lines of soybean. Plant Soil. J. 172: 131-139.

Cheema, M. A., Farhad, W. M. F. Saleem, H. Z. Khan, A. Munir, M. A. Wahid, F. Rasul. and H. M. Hammad. 2010. Nitrogen management strategies for sustainable maize production. Crop Environ. J. 1(1): 49-52.

Defreitas, J. R. 2000. Yield and N assimilation of winter wheat (Triticum aestivum L.) inoculated with rhizobacteria. Pedobiologia. J. 44: 97-104.

Echarte, L., F. H. Andrade, V. O. Sadras. and P. Abbat. 2006. Kernel weight and its response to source manipulations during seed filling in Argentinean maize hybrids released in different decades. Field Crops Res. J. 96(2): 307-312.

Fahramand, M. and H. R. Mobasser. 2013. Effect of biological fertilizer on maize. Intl. J. Agric. Crop Sci. 6(12): 784-788.

Gilik, B. R., D. Penrose. and M. Wenbo. 2001. Bacterial promotion of plant growth. Bio-tech. Advance. J. 19: 135–138.

Jalilian, J. and H. Delkhoshi. 2014. How much, leaves near the ear contribute on yield and yield components of maize. Cercetari Agronomice in Moldova. J. 47(2): 5-12.

Karadogan, T. and I. Akgun. 2009. Effect of leaf removal on sunflower yield and yield components and some quality characters. Helia. J. 32(50): 123-134.

Kizilkaya, R. 2008. Yield response and nitrogen concentration of spring wheat inoculated with Azotobacter chroococcum strains. Eco. Eng. J. 33: 150-156.

Lieth, J. H. and C. C. Pasian. 1990. A model for photosynthesis of rose leaves as a function of photosynthetic active radiation, leaf temperature and leaf age. J. Amer. Soc. Hort. Sci. 115: 486-491.

Lucy, M. and B. R. Glick. 2004. Application of free living plant growth promoting rhizobacteria. J. Antonie van Leeuwenhoek. 86: 1-25.

Meena, M. D., D. D. Tiwari, S. K. Chaudhari, D. R. Biswas, B. Narjary, A. L. Meena, B. L. Meena. and R. B. Meena. 2013. Effect of bio-fertilizer and level on yield and nutrient uptake by maize (Zea Maize L.). Annals Agri-Bio Res. 18(2): 176-181.

Mohammadi, G. R., M. E. Ghobadi. and S. Sheikheh-Poor. 2012. Phosphate Bio-fertilizer. Row spacing and plant density effects on corn (Zea mays L.) yield and weed growth. American J. Plant Sci. 3: 425-429.

Mouhamed, S. G. A. and S. A. H. Ouda. 2006. Predicting the role of some weather parameters on maize productivity under different defoliation treatments. J. Appl. Sci. Res. 2(11): 920-925.

Prioul, J. L. and N. S. Dugue. 1992. Kernel growth rate and duration in maize as effected by plant density and genotype. Crop Sci. J. 19: 385-388.

Richardsson, A. E. 2001. Prospects for using soil micro organisms to improve the acquisition of phosphorus by plants. Aust. J. Plant Physiol. 28: 897-906.

Saeidnejad, A. H. and P. Rezvani Moghadam. 2010. The effect of biological and chemical fertilizers on morphological characteristics, yield, yield components of corn. J. Hort. Sci. 24(1): 38-44.

Seilsepour, M., E. Baniani. and M. Kianirad. 2002. Effect of Phosphate Solubilizing Microorganism (PSM) in reducing the rate of phosphate fertilizers application to cotton crop. Proceed. 15th Intl. Meeting on Microbial Phosphate Solubilization Salamanca Univ. 16-19 July. Salamanca. Spain. pp: 20-24.

Shaharoona, B., M. Arshad, A. Z. Zahir. and A. Khalid. 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of corn (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biol. Bio. Chem. J. 38: 2971–2975.

Sharma, A. K. and B. N. Johri. 2002. Arbuscular Mycorrhizae: Interactions in Plants, Rhizosphere and soils. Sci. Pub. 311 p.

Soleimanzadeh, H. and F. Ghooshchi. 2013. Response of growth and yield of maize to bio-fertilizers in organic and conventional cropping systems. Int. J. Agric. Crop Sci. 5(7): 797-801.

Vadassery, J., C. Ritter, Y. Venus, I. Camehl, A. Varma, B. Shahollari, O. Novak, M. Strnad, J. Ludwig-Muller. and R. Oelmuller. 2008. The role of Auxins and Cytokinins in the mutualistic interaction between Arabidopsis and Piriformospora indica. American Phyto-pathological Soc. J. 21(10): 1371-1383.

Velmurugan, M., N. Chezhiyan. and M. Jawaharlal. 2008. Influence of organic manures and inorganic fertilizers on cured rhizome yield and quality of turmeric (Curcuma longa L.). BSR-2. Intl. J. Agric. Sci. 4(1): 142-145.

Vessy, K. 2003. Plant growth promoting rhizobacteria as biofertilizars. Plant and Soil. J. 255: 571-586.

Vissani, V., S. Rahimzadeh. and Y. Sohrabi. 2012. The effect of biological fertilizers on morphological, physiological traits and essence of medicinal plant of Ocimum basilicum L. J. Medic. Aromatic Plants Res. 28(1): 73– 87.

Wang, O. Q. 1996. Effects of altered source sink ratio on canopy photosynthetic rate and yield of maize. Photosynthetic. J. 32: 271- 267.

Yazdani, M., M. A. Bahmanyar, H. Pirdashti. and M. A. Esmaili. 2009. Effect of phosphate solubilization micro organisms and plant growth promoting rhizobacteria on yield and yield components of corn. Inter. J. Biol. Life Sci. 1: 2-11.

Zahir, A. Z., M. Arshad. and W. F. Frankenberger. 2004. Plant growth promoting rhizobacteria. App. Perspectives. Agric. Adv. Agron. 81: 97-168.

Zamir, M. S. I., G. Yasin, H. M. R. Javeed, A. U. H. Ahmad, A. Tanveer. and M. Yaseen. 2013. Effect of different sowing techniques and mulches on the growth and yield behavior of spring planted maize (Zea mays L.). Cercetari Agron. Moldova. 1(153): 77-82.

Zonuz, F. A., P. Gharavi Kouchebagh. and A. Gasemi. 2014. Effects of combined use of bio-fertilizer and chemical fertilizers at different stages on physiological and morphological character of bean. Indian J. Fund. App. Life Sci. 4(3): 423-427.